detection and enumeration of cryptosporidium oocysts in environmental water samples by real-time pcr assay

نویسندگان

mohammad-reza mahmoudi department of parasitology, faculty of medicine, guilan university of medical sciences, rasht, iran

mojgan bandepour cellular and molecular biology research center, shahid beheshti university of medical sciences, tehran, iran

bahram kazemi department of biotechnology, school of medicine, shahid beheshti university of medical sciences, tehran, iran

asad mirzaei department of parasitology, faculty of allied medical sciences, ilam university of medical sciences, ilam, iran

چکیده

introduction: the protozoan parasite, cryptosporidium spp., widely spreads in both raw and drinking waters. it is the causative agents of waterborne diarrhea and gastroenteritis in the world. in the present study, a molecular assay was used for the detection and quantification of cryptosporidium oocysts in environmental water samples. materials and methods: thirty surface water samples were collected from rasht city rivers and lagoons during 2009-2010. the samples were analysed for cryptosporidium oocysts using real time pcr method. samples were filtrated through a 1.2µm pore size cellulose nitrate membrane filter and then purified and quantified by real-time pcr technique. results: cryptosporidium oocysts were found in 19 of 30 the samples. oocyst concentration was ranging from 0.007 to 27 oocysts per liter of the examined waters. conclusion: the present study showed that the investigated water supplies were contaminated by cryptosporidium oocyst. this study indicated that in this level of oocysts there is a potential risk of waterborne cryptosporidiosis due to direct or indirect consumption of these waters by humans and animals. real-time pcr is a technique that provides high sensitivity for detection quantitative purposes.

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Detection and enumeration of Cryptosporidium oocysts in environmental water samples by Real-time PCR assay

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عنوان ژورنال:
journal of basic research in medical sciences

جلد ۴، شماره ۳، صفحات ۴۲-۴۷

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